Determination of modafinil in rat plasma by UPLC-MS/MS and a study of its pharmacokinetics and bioavailability

Abstract Modafinil has a strong and long-lasting awakening effect. Short-term use can improve cognitive work efficiency. Therefore, it been known to be abused by students parents as “smart drug.” It is in the first category of psychotropic drugs strictly controlled. To detect modafinil rat plasma study differences pharmacokinetics between oral sublingual administration rats, an ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed. Rats were injected with gavage vein, respectively, blood collected within certain period, obtained centrifugation. Midazolam used internal standard, concentration determined UPLC-MS/MS, where drug-time curve created calculate pharmacokinetic parameters. The standard for ranged from 1 2000 ng mL −1 good linearity. intra-day accuracy 86% 104%, 90% 103%. Intra-day precision (RSD%) less than 15%, inter-day 15%. matrix effect 93% 102%, recovery greater 91%. UPLC-MS/MS established this selectivity high sensitivity, successfully applied injection rats. bioavailability calculated 55.8%.